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Post by Rachael on Aug 26, 2004 11:12:19 GMT -5
I think it's kinda pretty. Hence my secretly liking it. It makes a potent earworm, though.
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Post by Sara on Aug 26, 2004 11:13:05 GMT -5
Ahem. Yes, but in my world, no one but me is waiting. And, often, if you can leave something overnight, you can leave it many overnights without ill effect. *shrug* Like the dishes. Believe me--I'm right there with you. Besides, you don't know yet that leaving the stuff in the fridge for that long was the problem, right?
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Post by Rachael on Aug 26, 2004 11:13:06 GMT -5
They're flies, not dishes. They're NOT flies. They're DNA ligations. They were flies a week ago. And also, There are currently no dirty dishes at my house.
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Post by Sara on Aug 26, 2004 11:14:19 GMT -5
Hence my secretly liking it. It makes a potent earworm, though. Seriously--I've had the song in my head since Erin's post with the title.
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Post by Rachael on Aug 26, 2004 11:15:40 GMT -5
Like the dishes. Believe me--I'm right there with you. Besides, you don't know yet that leaving the stuff in the fridge for that long was the problem, right? Nope. Coulda been the digestion that got left overnight rather than going straight to ligation, too. Although I inactivated the enzyme by heating it up, in theory, that seems like the more likely source of potential problems. Last week, I kept starting my molecular biology in the afternoon, and running up against the end of the day without enough time to do the next step. My brain told me that overnight refrigeration probably wouldn't hurt, but this is a little bit more sensitive an experiment than usual, so that "probably" looms large. Hell, one in six of them worked yesterday, anyway. So, in theory, I'm right. But only 1/6 of the time. ;D
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Post by Shan on Aug 26, 2004 11:17:33 GMT -5
They're NOT flies. They're DNA ligations. They were flies a week ago. And also, There are currently no dirty dishes at my house. Hee! Ok. Just...you know...thinking of the current state of my own house and shutting up because dishes are pretty much the only thing right now that's actually DONE.
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Post by Sara on Aug 26, 2004 11:18:52 GMT -5
Nope. Coulda been the digestion that got left overnight rather than going straight to ligation, too. Although I inactivated the enzyme by heating it up, in theory, that seems like the more likely source of potential problems. Last week, I kept starting my molecular biology in the afternoon, and running up against the end of the day without enough time to do the next step. My brain told me that overnight refrigeration probably wouldn't hurt, but this is a little bit more sensitive an experiment than usual, so that "probably" looms large. Hell, one in six of them worked yesterday, anyway. So, in theory, I'm right. But only 1/6 of the time. ;D Which puts you just under the Mendoza line, yes? ;D
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Post by Anne, Old S'cubie Cat on Aug 26, 2004 11:21:26 GMT -5
Yeah; that's one of those things that might indicate part death is imminent. Would that be a harbinger or a portent?
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Post by Matthew on Aug 26, 2004 11:22:36 GMT -5
Would that be a harbinger or a portent? I think that it's a porbinger.
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Post by MaryMuse on Aug 26, 2004 11:25:17 GMT -5
Now, see, Mary understands. No, really. That wasn't sarcasm. You're exactly right - it's vague because the answer is that it'd likely work in a couple hours, but works better after eight, and in this particular experiment, sometimes even better after the whole weekend. But the template I'm using is maybe a tad more unstable than other times, and it might have gotten chewed up by DNA autocatalysis. . .ignore that if you want, the technobabble at the end. Sounds intricate and fascinating. I way don't have the personality for detailed work like that. I'm a dash and a pinch cooker, I don't even really measure anything, and it turns out with no problems. But I know what you're doing is a bit more complicated than a batch of zucchini bread. ;D
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Post by Queen E on Aug 26, 2004 11:27:44 GMT -5
**runs screaming from the board** Well, we could do Candyland instead. Or Operation (that works even if you've lost some of the body parts). Ooohh! Or Life! I like playing that by just ramdomly jumping around the board doing what it says. And then we each pick a plastic building and pretend we live there and pick your favorite car color and pack it with as many different little people sticks as you want and pretend to drive all over and play house. OK? Yola We used to take the jump ropes and play horses instead. Shanno's reply: Talking of jump ropes, we used to get bags of colored rubber bands (or filch them from someone whose older brother had a paper round) and then loop (not tie) the rubber bands together until we had a chain of them long enough to play what we called "Chinese Jumprope" which was basically like a simplified Cat's Cradle but with your feet. Sometimes. Meh. I wanna go outside and play. Double-Dutch, anyone?
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Post by Sara on Aug 26, 2004 11:27:59 GMT -5
Would that be a harbinger or a portent? harbinger: 1 archaic: a person sent ahead to provide lodgings 2 a: one that pioneers in or initiates a major change: PRECURSOR b: one that presages or foreshadows what is to come portent: 1: something that foreshadows a coming event: OMEN 2: prophetic indication or significance 3: MARVEL, PRODIGY I think I'm going with portent.
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Post by Queen E on Aug 26, 2004 11:29:33 GMT -5
so not to lose the part while I make a new one.
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Post by Queen E on Aug 26, 2004 11:36:33 GMT -5
Long live the new part.
Go and enjoy.
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